{"id":20,"date":"2018-02-19T01:40:27","date_gmt":"2018-02-18T16:40:27","guid":{"rendered":"http:\/\/glycoaware.com\/tokai-u\/?page_id=20"},"modified":"2023-12-14T10:37:29","modified_gmt":"2023-12-14T01:37:29","slug":"welcome-new-home","status":"publish","type":"page","link":"http:\/\/glycoaware.com\/tokai-u\/","title":{"rendered":"Welcome to Kanie Lab at Tokai Univ"},"content":{"rendered":"

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Kanie Laboratory welcome graduate students from abroad. Please contact for more infomation. About 1000 students from all over the world are studying at Tokai University.<\/p>\n

We chemically synthesize carbohydrate-related molecules and use them in sensing elements against various chemical compounds. We also work on a project of miniature glycoprotein synthesis as a drug candidate.<\/p>\n

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\u751f<\/span>\u7269<\/span>\u5de5<\/span>\u5b66<\/span>\u79d1<\/span><\/span><\/a><\/h3>\n

2023\u5e743\u670819\u65e5\u3000\u30aa\u30fc\u30d7\u30f3\u30ad\u30e3\u30f3\u30d1\u30b9<\/strong><\/a>\u304a\u3053<\/strong>\u306a\u3044\u307e\u3057\u305f<\/strong><\/p>\n

\u5922\u30ca\u30d3\u30e9\u30a4\u30d6\u53c2\u52a0\u4e2d<\/a>\uff1a2023\u5e74\u590f\u300c\u7814\u7a76\u5ba4\u8a2a\u554f\u300d\u3092\u884c\u3044\u307e\u3057\u305f…\u9ad8\u6821\u751f\u306e\u7686\u3055\u3093\uff01<\/p>\n

\u5b66\u79d1\u306e\u30a4\u30e1\u30fc\u30b8\u6620\u50cf\uff08YouTube\uff09<\/a>\u516c\u958b\u4e2d<\/a>\u3067\u3059\uff01<\/p>\n

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Research Interests:<\/h3>\n

Living things developed various sensory mechanisms against electromagnetic field, gravitation, chemical entities. sound, etc. for their survival. We are interested in developing artificial sensory system for chemical compounds especially focusing on surface chemical properties of individual molecules and analytical methods. The type of mechanism may provide a universal type of sensor interface to detect molecules, viruses, and microbes.<\/p>\n

The followings are in Japanese. ===<\/p>\n

\u7814\u7a76\u30c6\u30fc\u30de<\/p>\n

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Regulatory mechanism of glycan synthesis inside cells…we used to work on…<\/h4>\n

Most of glycans are synthesized at ER and Golgi apparatus by cascade reactions of a series of glycosyltransferases utilizing sugar nucleotides, most of which are synthesized in cytosol and transported into Golgi rumen, however, the mechanism how such synthesis is controlled is not known. We address this issue by using synthetic probe molecule that carries reporter functionality. In particular, we utilize lactosyl ceramide (LacCer) analogs carrying BODIPY dyes. LacCer is located at the fork in a complex glycosphingolipid synthetic pathway. We combine chemistry and analytical chemistry to synthesize probe molecules and to analyze glycan transformation.<\/p>\n

Due to a situation, we had to take a break on this subject, but we hope someone pursuit the type of research to understand the glycan processing mechanism in Golgi apparatus somewhere…<\/p>\n

A list of our papers associated to this subject.<\/h4>\n
    \n
  1. Sequential enzymatic glycosyltransfer reactions on a microfluidic device: Synthesis of a glycosaminoglycan linkage region tetrasaccharide.\u00a0<\/a>Ono Y, Kitajima M, Daikoku S, Shiroya T, Nishihara S, Kanie Y, Suzuki K, Goto S, Kanie O.
    \nLab Chip.<\/strong><\/em> 2008<\/strong> Dec;8(12):2168-73. doi: 10.1039\/b809316d.\u00a0 IF 7.5
    \n<\/strong><\/li>\n
  2. Mechanism of a gas-phase dissociation reaction of 4-aminobutyl glycosides under CID MS\/MS conditions.\u00a0<\/a>Shioiri Y, Suzuki K, Kanie O.
    \nJ Mass Spectrom.<\/strong><\/em> 2008<\/strong> Aug;43(8):1132-9. doi: 10.1002\/jms.1397.\u00a0 IF 2.4<\/strong><\/li>\n
  3. N-Hexyl-4-aminobutyl glycosides for investigating structures and biological functions of carbohydrates.\u00a0<\/a>Suzuki K, Tobe A, Adachi S, Daikoku S, Hasegawa Y, Shioiri Y, Kobayashi M, Kanie O.
    \nOrg Biomol Chem.<\/strong> <\/em>2009<\/strong> Nov 21;7(22):4726-33. doi: 10.1039\/b909556j.\u00a0 IF 3.9<\/strong><\/li>\n
  4. Energy-Resolved Structural Details Obtained from Gangliosides.\u00a0<\/a>Shioiri Y, Kurimoto A, Ako T, Daikoku S, Ohtake A, Ishida H, Kiso M, Suzuki K, Kanie O.
    \nAnal Chem.<\/strong><\/em> 2009<\/strong> Jan 1;81(1):139-45. doi: 10.1021\/ac801611z.\u00a0 IF 8.0<\/strong><\/li>\n
  5. Fluorescence-monitored zero dead-volume nanoLC-microESI-QIT-TOF MS for analysis of fluorescently tagged glycosphingolipids.\u00a0<\/a>Daikoku S, Ono Y, Ohtake A, Hasegawa Y, Fukusaki E, Suzuki K, Ito Y, Goto S, Kanie O.
    \nAnalyst.<\/strong><\/em> 2011<\/strong> Mar 7;136(5):1046-50. doi: 10.1039\/c0an00715c.\u00a0 IF 5.2<\/strong><\/li>\n
  6. Synthesis of a fluorescently tagged sialic acid analogue useful for live-cell imaging.\u00a0<\/a>Suzuki K, Ohtake A, Ito Y, Kanie O.
    \nChem Commun (Camb).<\/strong><\/em> 2012<\/strong> Oct 9;48(78):9744-6. doi: 10.1039\/c2cc34605b.\u00a0 IF 6.1<\/strong><\/li>\n
  7. Analysis of the Cellular Dynamics of Fluorescently Tagged Glycosphingolipids by Using a Nanoliquid Chromatography-Tandem Mass Spectrometry Platform.\u00a0<\/a>Ohtake A, Daikoku S, Suzuki K, Ito Y, Kanie O.
    \nAnal Chem.<\/strong><\/em> 2013<\/strong> Sep 17;85(18):8475-82. doi: 10.1021\/ac401632t.\u00a0 IF 8.0<\/strong><\/li>\n
  8. Syntheses of lactosyl ceramide analogues carrying novel bifunctional BODIPY dyes directed towards the differential analysis of multiplexed glycosphingolipids by MS\/MS using iTRAQ.\u00a0<\/a>Son SH, Daikoku S, Ohtake A, Suzuki K, Kabayama K, Ito Y, Kanie O.
    \nChem Commun (Camb).<\/strong><\/em> 2014<\/strong> Mar 21;50(23):3010-3. doi: 10.1039\/c4cc00112e.\u00a0 IF 6.1<\/strong><\/li>\n
  9. Synthetic study of 3-fluorinated sialic acid derivatives.\u00a0<\/a>Suzuki K, Daikoku S, Son SH, Ito Y, Kanie O.
    \nCarbohydr Res.<\/strong><\/em> 2015<\/strong> Apr 10;406:1-9. doi: 10.1016\/j.carres.2014.12.010.\u00a0 IF 3.0
    \n<\/strong><\/li>\n
  10. The relationship between glycan structures and expression levels of an endoplasmic reticulum-resident glycoprotein, UDP-glucose: Glycoprotein glucosyltransferase 1.\u00a0<\/a>Daikoku S, Seko A, Son SH, Suzuki K, Ito Y, Kanie O.
    \nBiochem Biophys Res Commun.<\/strong><\/em> 2015<\/strong> Jun 19;462(1):58-63. doi: 10.1016\/j.bbrc.2015.04.105.\u00a0 IF 3.3<\/strong><\/li>\n
  11. Evaluation of reversed-phase nano liquid chromatography conditions by using reversed-phase thin layer chromatography based on Hansen solubility parameters for the analysis of amphiphilic glycosylsphingolipid transformations.\u00a0<\/a>Kanie Y, Taniuchi M, Kanie O.
    \nJ Chromatogr A.<\/strong><\/em> 2018<\/strong> Jan 26;1534:123-129. doi: 10.1016\/j.chroma.2017.12.058.\u00a0 IF 4.6<\/strong><\/li>\n<\/ol>\n

    The program now terminates.<\/p>\n

    \"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

    Kanie Laboratory welcome graduate students from abroad. Please contact\u2026<\/p>\n

    Continue reading<\/span><\/i><\/a> <\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-20","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/pages\/20","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/comments?post=20"}],"version-history":[{"count":32,"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/pages\/20\/revisions"}],"predecessor-version":[{"id":1004,"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/pages\/20\/revisions\/1004"}],"wp:attachment":[{"href":"http:\/\/glycoaware.com\/tokai-u\/wp-json\/wp\/v2\/media?parent=20"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}